Thermolysin-catalyzed peptide bond synthesis.
نویسندگان
چکیده
منابع مشابه
Thermolysin-catalyzed peptide bond synthesis.
The rates of the thermolysin-catalyzed synthesis of peptides have been determined by means of HPLC. In the condensation of various N-substituted amino acids and peptides with L-leucinanilide, the enzyme exhibits preference for a hydrophobic L-amino acid as the donor of the carbonyl group of the newly formed bond. The presence of another hydrophobic amino acid residue adjacent to the carbonyl-gr...
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The early studies on proteinase-catalyzed peptide bond synthesis are summarized as background for a report on some recent work in our laboratory on pepsin and thermolysin. The primary and secondary specificity of these two enzymes have been found to be suitable for the preparative synthesis of oligopeptides by the condensation of two hydrophobic L-amino acid residues. Kinetic data are presented...
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It is demonstrated that carboxypeptidase Y from Saccharomyces cerevisiae can catalyze the formation of peptide bonds using N-acylamino acid esters as substrates and free amino acids or amino acid amides as nucleophiles, The coupling yields observed with free amino acids were max. 60 % for alanine and lysine and they depended strongly on the reaction parameters; viz. pH, temperature and concentr...
متن کاملRegioselectivity in acylation of oligosaccharides catalyzed by the metalloprotease thermolysin
Investigation of the acylation scope of carbohydrates by metalloprotease thermolysin immobilized on Celite as biocatalyst has been carried out. The reactions were performed in DMSO, a good solvent for carbohydrates, where the enzyme has previously shown its activity in transesterifications of sucrose, maltose and maltose-containing oligosaccharides. Surprisingly, no reaction was observed for gl...
متن کاملEffect of Microwave Radiation on Enzymatic and Chemical Peptide Bond Synthesis on Solid Phase
Peptide bond synthesis was performed on PEGA beads under microwave radiations. Classical chemical coupling as well as thermolysin catalyzed synthesis was studied, and the effect of microwave radiations on reaction kinetics, beads' integrity, and enzyme activity was assessed. Results demonstrate that microwave radiations can be profitably exploited to improve reaction kinetics in solid phase pep...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.11.3241